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Facilitator QuestionsWhy has dynein been harder to study than kinesin? Why did the Vale lab choose yeast as a model system? What type of experiment allowed the investigators to show that dynein was processive? What is the difference in the stepping behavior of kinesin and dynein? How did the Vale lab show that dynein is processive and requires two motor domains? Why does yeast dynein need to be processive and produce large force? What evidence do the investigators present that the two dynein motor domains shuffle past one another (like walking legs)?
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