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Review questions

 

  1. What is a coat protein complex and what is its role in vesicle transport?

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  2. What is meant by “anterograde” and “retrograde” transport with respect to vesicle transport between the ER and Golgi? Which coat protein complex is involved in each of these two types of transport?

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  3. From where do vesicle membranes originate?

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  4. What role do SNARE proteins play in vesicle trafficking?

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  5. What would happen if transport from the ER to Golgi were disrupted? What if transport from the Golgi to the ER were disrupted?


  6. With what efficiency can vesicles be separated from intact ER membranes in vitro? How is this achieved?

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  7. How was it experimentally shown that the proteins involved in COPII vesicle formation actually form a coat complex around vesicles?

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  8. What was the point of conducting COPII budding assays using yeast nuclei? Why were yeast nuclei used as an experimental source of ER membrane?

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  9. What triggers COPII coat assembly, and how is this event specific to the ER?

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  10. What happens to the coat complex proteins after a COPII-coated vesicle has budded?